GroEL
Review of cryo EM
basic principles: Orlova
& Saibil, Chem Rev 2011 The
Saibil group studies the
operation of macromolecular
machines using three-dimensional
electron microscopy. Our
research projects are in the
areas of molecular chaperones
and assisted protein
folding/unfolding, misfolding
into amyloid, and protein
refolding in membrane pore
formation. On the cellular side,
we are studying these processes
in host cell interactions with
intracellular pathogens. Our
studies use single particle
cryo-electron microscopy and
electron tomography of isolated
complexes, irregular assemblies
and cell sections. In
collaborative work, we combine
these EM studies with
fluorescence and atomic force
microscopies.
