Data detail |
| info Data name | Details of rice EST mapping results |
| info DOI | 10.18908/lsdba.nbdc00318-04-002 |
| info Description of data contents | The data contains the detailed results of PCR-based YAC screening with the clone-specific EST primers. |
| info Data file |
File name: rgp_estmap2001_detail.zip
|
| info Simple search URL | http://togodb.biosciencedbc.jp/togodb/view/rgp_estmap2001_detail#en |
| info Data acquisition method | We used clone-specific primer pairs designed from 6713 unique EST sequences (3' end) derived from 19 cDNA libraries. |
| info Data analysis method | SEGMAP (version 3.48) was used for the analysis of YAC screening data and the establishment of the EST map.
DNA gel blot hybridization of YAC or rice genomic DNA with genetic markers or EST clones was conducted using the enhanced chemiluminescence system (Amersham, Buckinghamshire, UK).
Notes
#; floating markers on the genetic map
c; chimeric YAC clones
*; PCR band size longer than predicted
Ann T; 60 °C unless otherwise indicated
NI; YACs with unknown insert |
| info Number of data entries | 6,760 entries |
| Data item | Description |
|---|
| Anchor |
Genetic markers used for YAC landing |
| Chrom. No. |
Chromosome number |
| Position (cM) |
Genetic distance (cM) |
| EST Clone |
EST Clone (sites) name |
| DDBJ Acc FW |
DDBJ accession of the EST clone (Forward sequence) |
| DDBJ Acc RV |
DDBJ accession of the EST clone (Reverse sequence) |
| Positive YACs |
Positive YAC names |
| PCR Primer |
PCR primer name |
| Amplified Band Size |
Amplified PCR band size (kb) |
| Ann T |
Annealing temperature (°C) |
| 5'-Primer Sequence |
Primer sequence (5' primer, each 20 bases) |
| 3'-Primer Sequence |
Primer sequence (3' primer, each 20 bases) |
| Map Image File |
Image file name of the rice transcript map |
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