Life Science Database Archive
eSOL

Solubility

Data detail

info Data name Solubility
info DOI
info Description of data contents

The solubilities of individual proteins were evaluated by using a cell-free translation system (PURE system) which is chaperone-free.
The data of chaperone effects on the prevention of aggregate formation are available for 788 aggregation-prone proteins.

The concentrations of chaperones were as follows:
- TF (Trigger factor): 5.0 (monomer) μM
- GroE (GroEL and GroES): 0.5 (tetradecamer) and 1.0 (heptamer) μM
- KJE (DnaK, DnaJ and GrpE): 5.0 (monomer), 2.0 (monomer), and 2.0 (monomer) μM

info Data file
File name:
esol.zip
File size:
192 KB
info Simple search URL
info Data acquisition method

After the protein synthesis with [35S]methionine, an aliquot was withdrawn as the total fraction, and the remainder was centrifuged at 21,600 ×ばつ g for 30 min. Both the total and supernatant fractions were separated by SDS-PAGE, and the band intensities were quantified by autoradiography.

info Data analysis method

-

info Number of data entries

4,132 entries. (Out of all the entries, 3,173 entries have the data of solubilities and 788 entries have the data of chaperone effects on the prevention of aggregate formation.)

Data itemDescription
JW_ID Gene ID assigned by GenoBase to the E. coli strain K-12 substrain W3110.
ECK number

Accession number which can refer to genes in both substrains MG1655 and W3110 of the E. coli strain K-12.

B number Gene accession number assigned by the Blattner laboratory to the E. coli strain K-12 substrain MG1655.
Gene name K-12 Gene name of the E. coli strain K-12.
Locus name K-12 Locus name of the E. coli strain K-12.
Synonyms of locus names K-12 Synonyms of the locus name of the E. coli strain K-12.
Solubility (%) Solubility determined in Niwa et. al., PNAS 2009. (unit: %)
Yield (uM) Yield of the protein measured in Niwa et. al., PNAS 2009. (unit: μM)
Yield (ug/ml) Yield of the protein measured in Niwa et. al., PNAS 2009. (unit: μg/ml)
Minus Sol (%) Solubility in the absence of any chaperons. (unit: %) It is redetermined to evaluate chaperon effects.
TF Sol (%) Solubility in the presence of Trigger Factor. (unit: %)
GroE Sol (%) Solubility in the presence of GroEL/ES. (unit: %)
KJE Sol (%) Solubility in the presence of DnaK/DnaJ/GrpE. (unit: %)
Minus Yield (uM) Yield in the absence of any chaperons. (unit: μM)
TF Yield (uM) Yield in the presence of Trigger Factor. (unit: μM)
GroE Yield (uM) Yield in the presence of GroEL/ES. (unit: μM)
KJE Yield (uM) Yield in the presence of DnaK/DnaJ/GrpE. (unit: μM)
Minus Yield (ug/ml) Yield in the absence of any chaperons. (unit: μg/ml)
TF Yield (ug/ml) Yield in the presence of Trigger Factor. (unit: μg/ml)
GroE Yield (ug/ml) Yield in the presence of GroEL/ES. (unit: μg/ml)
KJE Yield (ug/ml) Yield in the presence of DnaK/DnaJ/GrpE. (unit: μg/ml)
Calculated MW (kDa) Molecular weight calculated from amino acid sequences. (unit: kDa)
Calculated pI Isoelectric point calculated from amino acid sequences.
Type of gene product Type of gene product obtained from Genobase.
(About the description of "Type of gene product", see this page.)
Gene product description Description obtained from Genobase.
Cell location Localization in the cell.
Structure (PDB) ID PDB ID obtained from Genobase.
SCOP assignment SCOP assignment obtained from Genobase.

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