Sigmoidal model fitting, optimal model selection and calculation of various features that are essential in the analysis of quantitative real-time polymerase chain reaction (qPCR), according to Ritz & Spiess (2008) <doi:10.1093/bioinformatics/btn227> and Spiess et al. (2008) <doi:10.1186/1471-2105年9月22日1>.
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