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I created a thread, and started it and then called its run method. It raised an AttributeError exception
from threading import Thread
def func():
 print 'abc'
t = Thread(None, func)
t.start()
t.run()
here t.run() raises an exception.

Don't call both start() and run(). From the documentation, start() arranges for run() to be called. After the call to start(), 'abc' is printed.

I am also not able to call run() twice. I have python 2.5.2 with me, in which I am able to call run method twice and calling run after start is working.

May be this is a behavior change, that a thread can not be run again. I think documentation needs to be changed in this case.

While it's not explicitly documented that run() also shouldn't be called multiple times, it does not need to be supported. Threads can be started exactly once -- this is already mentioned in the docs.
Note that run() simply calls the thread target with the given args. Calling thread.run() instead is just a more confusing way of doing this.